Introduction: Job stress in nursing staff is an effective factor that decrease job competency and resulted to nursing care delivery non- effectively. But, applying spiritual intelligence could be decreased job stress and resulted to job satisfaction. Aim: This study was done for determining effects spiritual intelligence training on nurses' job stress. Methods and Materials: this study was one group pre and post experimental and control group design that was done 2014. Sample size was (94) nurses who worked in Vali- e- Aser Hospital in Birjand province. They were selected based on units and then allocated to two groups (case/ control) randomly. Data gathering was done by nurses stress scale's Taft and Anderson (1981) (NSS). After twelve training workshops for case group for two months data gathered immediately after the end of workshop and after one month in two groups' pre and post intervention. Data were analyzed by descriptive and inferential statistics tests (ANOVA one way, t- test, repeated measurement) in SPSS16. Results: findings showed that two groups were matched and there was no significant difference between two groups before intervention (P = 0.68) in their job stress. After intervention, the mean of job stress decreased in case group (p &le 0.05) so, before intervention mean of job stress was (44/91± 11/22), and after intervention was (40/43 ± 9/06). ANOVA test in control group showed that mean job stress before, after and one month after intervention no changed (p = 0.67). But this repeated measurement test in case group showed that the mean of job stress were decreased significantly (p = 0.02). Conclusion: Because of job stress is situational, it may be after long time training changed and increased, and so, training must be continued all over time by nurse managers.